Chongqing Peg-Bio Biopharm Co., Ltd.

1 Product Details

Name：Recombinant Trypsin （rTP） 

CAS：9002-07-7                               Brand：IMED

Origin: Chongqing, China Storage conditions: -15°C

Purity: β-trypsin not less than 70% Product category: Recombinant protease

Product Grade: Pharmaceutical Grade Alias: Trypsin

Specification: 1mg/bottle, 10mg/bottle, 0.5g/bottle Main uses: rTP is widely used in the production of recombinant insulin and its analogues, GLP-1 and its analogues, etc.

2 Product Description

Trypsin (EC: 3.4.21.4) is a serine protease that specifically cleaves the carboxy-terminal peptide bonds of Lys or Arg. The recombinant trypsin (rTP) produced by Chongqing IMED was prepared by recombinantly expressing Picrosporum yeast, and the amino acid sequence was consistent with that of porcine trypsin. The rTP cleavage pH ranged from 5.0-9.0 and the optimal reaction pH was 7.6-8.5. Its biological activity is inhibited by serine protease inhibitors such as DFP, PMSF, and TLCK.

3 Quality Standards

4 Product Features

• The product quality meets the quality standards of the 2020 edition of the People's Republic of China Pharmacopoeia and USP 38th edition;

• Recombinant expression preparation, no animal-derived materials are used in the production process, and there is no risk of exogenous virus contamination;
  

• 3000L fermentation scale production line, with an annual output of not less than 15kg; High batch-to-batch quality consistency through multi-batch production validation;

• The production workshop is operated according to GMP, and the production equipment, environment and process control are implemented with reference to the Good Manufacturing Practice (GMP);

• Provide matching rTP protease trace residue detection kit;

• Relevant regulatory support documents can be provided according to customer requirements;

• The packaging specifications can be customized according to customer requirements.

5 Product Applications

In the field of biopharmaceutical industry, rTP is widely used in the production of recombinant insulin and its analogues, and the production of recombinant peptide fusion expression such as GLP-1 and its analogues. various cell cultures, such as vaccine production, stem cell isolation, etc.; Production of small molecule proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptides. In the field of scientific research, rTP is used in proteomics research, peptide sequencing, phosphorylated peptide identification, disulfide bond analysis, N-glycan analysis, etc.

6 Packing and Shipping

This product is in the form of lyophilized powder, and is packaged in HDPE bottles for oral use, which is convenient for transportation and storage; It can be stored at -15 °C for at least 2 years. The whole process of cold chain transportation, the whole process of temperature monitoring from outbound to receiving.