Chongqing Peg-Bio Biopharm Co., Ltd.

1 Product Information

Name:Recombinant Enterokinase (EK)  Brand:IMED

Place of origin: Chongqing, China Storage conditions: -15°C

Purity: 99% Product Category: Recombinant Protease

Grade: Pharmaceutical Grade Alias: Enterokinase

Specification: 50μg, 1mg, 10mg/piece; 1g, 10g, 50g/bottle Main uses: It can specifically recognize and digest the carboxy-terminus of DDDDK or DEGDR sequences, or for the removal of FLAG peptides in N-terminal and Met-N-terminal fusion proteins, and is widely used in the production and scientific research fields of recombinant proteins and peptides in biopharmaceuticals.

2 Products

Enterokinase (EC: 3.4.21.9) specifically recognizes and cleaves the carboxy terminus of DDDDK or DEGDR sequences. Recombinant enterokinase (rEK) produced by Chongqing IMED is a recombinant bovine enterokinase light chain fragment (containing 6× His tag) expressed by Pichia pastoris. rEK cleavage pH ranged from 4.5 to 9.5, with an optimal pH of 8.0; No reduction in enzyme specific activity was observed in 2 mol/L urea or 0.1% SDS or 100 mmol/L imidazole solution.

3 Quality Standards

4 Product Features

• The product is in the form of lyophilized powder, which can be stably stored at -15°C for at least 2 years;

• Recombinant expression preparation, no animal-derived materials are used in the production process, and there is no risk of exogenous virus contamination;

• 3000L fermentation scale production line, with an annual output of not less than 3kg; High batch-to-batch quality consistency through multi-batch production validation;

• The production workshop is operated according to GMP, and the production equipment, environment and process control are implemented with reference to the Good Manufacturing Practice (GMP);

• Provide matching rEK protease trace residue detection kit;

• Relevant regulatory support documents can be provided according to customer requirements;

• The packaging specifications can be customized according to customer requirements.

5 Product Applications

rEK is mainly used for enzymatic cleavage of fusion proteins containing DDDDK or DEGDR bonds, or for the removal of FLAG peptides from N-terminal and Met-N-terminal fusion proteins, and is widely used in the production and manufacturing of recombinant proteins and peptides in biopharmaceuticals and scientific research.

6 Packaging & Shipping

This product is in the form of lyophilized powder, and is packaged in HDPE bottles for oral use, which is convenient for transportation and storage; It can be stored at -15 °C for at least 2 years. The whole cold chain transportation, temperature monitoring from delivery to receipt.

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